Kerk aan de Kolk (Church at the Kolk) in Delfshaven, Rotterdam. The Kolk is the water on the foreground. The church's claim to fame is that night in July 1620, when the Pilgrim Fathers knelt down in prayer on the quay near the church before boarding the Speedwell. The church is now known as the Pilgrim Fathers' Church. The church's website has more information on the history of the church (also in English).
I visited Delfshaven a few years ago. I posted my photos last year to Trace your Dutch roots.